Western blot analysis of extracts of 293T cells using Phospho-AMPKα1(S485) Polyclonal Antibody at dilution of 1:1000. 293T cells were treated by oligomycin (0. 5 uM) at 37°C for 30 minutes.
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen Information
Immunogen
A phospho specific peptide corresponding to residues surrounding S485 of human PRKAA1