Phospho-CFL1(S3) Polyclonal Antibody
Catalog Number
RD268106A
Western blot analysis of extracts of NIH/3T3 cells using Phospho-CFL1(S3) Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue, using Phospho-CFL1(S3) Polyclonal Antibody
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-CFL1(S3) Polyclonal Antibody
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
Phospho-CFL1(S3) Polyclonal Antibody
Catalog Number
RD268106A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Immunogen Information
Immunogen
A phospho specific peptide corresponding to residues surrounding S3 of human CFL1
Gene ID
1072
Swissprot
P23528
Synonyms
CFL1CFLHEL-S-15cofilincofilin-1
Calculated MW
18 kDa
Observed MW
18 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:100-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas