Phospho-eNOS(S1177) Polyclonal Antibody
Catalog Number
RD268168A
Western blot analysis of extracts of SKOV3 cells using Phospho-eNOS(S1177) Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Mouse spinal cord using Phospho-eNOS(S1177) Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse pancreas using Phospho-eNOS(S1177) Polyclonal Antibody at dilution of 1:200 (40x lens).
Variation
Quantity
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Overview
Product Name
Phospho-eNOS(S1177) Polyclonal Antibody
Catalog Number
RD268168A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Immunogen Information
Immunogen
A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).
Gene ID
4846
Swissprot
P29474
Synonyms
NOS3ECNOSeNOS
Calculated MW
67 kDa/68 kDa/133 kDa
Observed MW
133 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas