Western blot analysis of extracts of NIH/3T3 cells using Phospho-HDAC5(S498) Polyclonal Antibody at dilution of 1:2000. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.
Immunohistochemistry of paraffin-embedded Human breast carcinoma using Phospho-HDAC5(S498) Polyclonal Antibody
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A phospho specific peptide corresponding to residues surrounding S498 of human HDAC5