Phospho-MAP2K1(S298) Polyclonal Antibody
Catalog Number
RD268066A
Western blot analysis of extracts of various cell lines using Phospho-MAP2K1(S298) Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using Phospho-MAP2K1(S298) Polyclonal Antibody at dilution of 1:1000. C2C12 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or treated by ATP(5 mM) at 30°C for 1 hour. C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or treated by ATP(5 mM) at 30°C for 1 hour.
Variation
Quantity
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Overview
Product Name
Phospho-MAP2K1(S298) Polyclonal Antibody
Catalog Number
RD268066A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Immunogen Information
Immunogen
A synthetic phosphorylated peptide around S298 of human MAP2K1 (NP_002746.1).
Gene ID
5604
Swissprot
Q02750
Synonyms
CFC3MAPKK1MEK1MKK1PRKMK1MAP2K1
Calculated MW
40 kDa/43 kDa
Observed MW
43 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas