Western blot analysis of extracts of K-562 and MCF-7 cells using Phospho-MEF2C(S396) Polyclonal Antibody at dilution of 1:1000. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Immunofluorescence analysis of U2OS cells using Phospho-MEF2C(S396) Polyclonal Antibody
This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described.
Immunogen Information
Immunogen
A synthetic phosphorylated peptide around S396 of human MEF2C (NP_002388.2).