Phospho-NF-kB p65(S536) Polyclonal Antibody
Catalog Number
RD268158A
Western blot analysis of extracts of HeLa cells using Phospho-NF-kB p65(S536) Polyclonal Antibody at dilution of 1:1000. HeLa cells were treated by TNF-α (20ng/ml) for 30 minutes.
Immunohistochemistry of paraffin-embedded Mouse testis using Phospho-NF-kB p65(S536) Polyclonal Antibody at dilution of 1:200 (40x lens).
Variation
Quantity
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Overview
Product Name
Phospho-NF-kB p65(S536) Polyclonal Antibody
Catalog Number
RD268158A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A phospho specific peptide corresponding to residues surrounding S536 of human NF-kB p65
Gene ID
5970
Swissprot
Q04206
Synonyms
NFKB3p65NF-kB p65RELACMCU
Calculated MW
58 kDa/59 kDa/60 kDa
Observed MW
71 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000 IHC 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.