Phospho-P70S6K1(T389) Polyclonal Antibody
Catalog Number
RD268240A
Western blot analysis of extracts of 293T cells using Phospho-P70S6K1(T389) Polyclonal Antibody at dilution of 1:1000. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. 293T cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.
Variation
Quantity
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Overview
Product Name
Phospho-P70S6K1(T389) Polyclonal Antibody
Catalog Number
RD268240A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Immunogen Information
Immunogen
A phospho synthetic peptide corresponding to residues surrounding T389 of human P70S6K1.
Gene ID
6198
Swissprot
P23443
Synonyms
PS6KS6KS6K-beta-1S6K1STK14Ap70 S6KAp70(S6K)-alphap70-S6Kp70-alphaP70 S6KRPS6KB1p70S6KA
Calculated MW
59 kDa
Observed MW
85 kDa/90 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.