Phospho-PAK1 (Thr212) Polyclonal Antibody
Catalog Number
RD86167A
Western Blot analysis of 293T cells with Phospho-PAK1 (Thr212) Polyclonal Antibody
Variation
Quantity
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Overview
Product Name
Phospho-PAK1 (Thr212) Polyclonal Antibody
Catalog Number
RD86167A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Immunogen Information
Immunogen
Synthesized peptide derived from human PAKα around the phosphorylation site of Thr212
Swissprot
Q13153
Synonyms
ADRB2Alpha PAKAlpha-PAKMGC130000MGC130001p21 activated kinase 1p21 protein (Cdc42/Rac) activated kinase 1p21-activated kinase 1p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related)p21/Cdc42/Rac1-activated kinase 1 (STE20 homologyeast)p65 PAK
Calculated MW
61 kDa
Observed MW
65 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas