Phospho-SMAD3(T179) Polyclonal Antibody
Catalog Number
RD268181A
Western blot analysis of extracts of PC-12 cells using Phospho-SMAD3(T179) Polyclonal Antibody at dilution of 1:1000. PC-12 cells were treated by TNF-α (20 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Western blot analysis of extracts of HeLa cell line using Phospho-SMAD3(T179) Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Human colon using Phospho-SMAD3(T179) Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using Phospho-SMAD3(T179) Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Rat liver using Phospho-SMAD3(T179) Polyclonal Antibody at dilution of 1:200 (40x lens).
Variation
Quantity
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Overview
Product Name
Phospho-SMAD3(T179) Polyclonal Antibody
Catalog Number
RD268181A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Immunogen Information
Immunogen
A synthetic phosphorylated peptide around T179 of human SMAD3 (NP_005893.1).
Gene ID
4088
Swissprot
P84022
Synonyms
HSPC193HsT17436JV15-2LDS1CLDS3MADH3SMAD3
Calculated MW
25 kDa/35 kDa/43 kDa/48 kDa
Observed MW
48 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.