Western blot analysis of extracts of various cell lines using Phospho-SMC1A(S957) Polyclonal Antibody at dilution of 1:1000. HeLa and NIH/3T3 cells were treated by UV for 15-30 minutes.
Western blot analysis of extracts of HeLa cells using Phospho-SMC1A(S957) Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of U2OS cells using Phospho-SMC1A(S957) Polyclonal Antibody
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Phospho-SMC1A(S957) Polyclonal Antibody
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen Information
Immunogen
A synthetic phosphorylated peptide around S957 of human SMC1A (NP_006297.2).