PRPF8 Polyclonal Antibody
Catalog Number
RD83617A
Western blot analysis of extracts of various cell lines using PRPF8 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human esophagus using PRPF8 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using PRPF8 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse stomach using PRPF8 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using PRPF8 Polyclonal Antibody at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
PRPF8 Polyclonal Antibody
Catalog Number
RD83617A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
Immunogen Information
Immunogen
A synthetic peptide of human PRPF8 (NP_006436.3).
Gene ID
10594
Swissprot
Q6P2Q9
Synonyms
PRPF8HPRP8PRP8PRPC8RP13SNRNP220PRPF8
Calculated MW
273 kDa
Observed MW
274 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas