SIGLEC3/CD33 Polyclonal Antibody
Catalog Number
RD82501A
Western blot analysis of extracts of various cell lines using SIGLEC3/CD33 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Mouse spleen using SIGLEC3/CD33 Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of THP-1 cells using SIGLEC3/CD33 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
SIGLEC3/CD33 Polyclonal Antibody
Catalog Number
RD82501A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
Immunogen Information
Immunogen
Recombinant fusion protein of human SIGLEC3/CD33 (NP_001763.3).
Gene ID
945
Swissprot
P20138
Synonyms
CD33SIGLEC-3SIGLEC3p67
Calculated MW
25 kDa/33 kDa/39 kDa
Observed MW
70 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas