SMAD7 Polyclonal Antibody
Catalog Number
RD265776A
Western blot analysis of extracts of SW480 cells using SMAD7 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of U2OS cells using SMAD7 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
SMAD7 Polyclonal Antibody
Catalog Number
RD265776A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human SMAD7 (NP_005895.1).
Gene ID
4092
Swissprot
O15105
Synonyms
SMAD7CRCS3MADH7MADH8
Calculated MW
23 kDa/46 kDa
Observed MW
46 kDa
Applications
Reactivity
Human
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas