Antibody Applications Guide

Antibody Applications Guide

Apr 30, 2024

Use this guide to better understand what applications antibodies can be used for. 

Abbreviationspace

Term

Definition

Activ

Activation

Inducing a biological response in cells or organisms using antibodies.

Apoptosis

Programmed cell death process; antibodies can detect or induce apoptosis.

Binding

Specific attachment of an antibody to its target antigen.

Block

Blocking

Inhibiting a specific biological interaction or activity using antibodies.

Cell Sep-Neg

Cell Separation by Negative Selection

Removing unwanted cells using antibodies targeting specific markers.

Depletion

Reducing or removing specific cell populations or molecules with antibodies.

ELISA

Enzyme-linked immunosorbent assay

Biochemical assay to detect proteins using antibodies for capture and detection.

FA

Functional Assay

Experiment to assess the functional activity of proteins using antibodies.

FCM

Flow Cytometry

Analyzing and sorting cells based on properties, often using fluorescent antibodies.

ICC

Immunocytochemistry

Visualizing proteins in cells using fluorescently labeled antibodies.

ICFCM

Intracellular Staining for Flow Cytometry

Detecting intracellular proteins in flow cytometry using antibodies after cell permeabilization.

IF

Immunofluorescence

Microscopy technique using fluorescent antibodies to visualize proteins in tissues/cells.

IHC

Immunohistochemistry

Visualizing proteins in tissue samples using antibodies.

IHC-p

Immunohistochemistry-Paraffin

IHC technique for formalin-fixed, paraffin-embedded tissue samples.

Inhibition

Blocking or suppressing a biological process using antibodies.

IP

Immunoprecipitation

Isolating specific proteins from a mixture using antibodies.

Neut

Neutralization

Rendering biological agents ineffective by blocking their activity with antibodies.

Ready To Use For IHC-P

Pre-optimized antibodies for immediate use in IHC on paraffin-embedded tissues.

Stim

Stimulation

Inducing or enhancing a biological response using antibodies.

WB

Western Blotting

Detecting and analyzing proteins based on size and charge using antibodies.

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